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Wagner Fontes

Wagner Fontes, MD, PhD

Neutrophil Proteomics Research Group

University of Brasilia
Dept. of Cell Biology
Laboratory of Biochemistry and Protein Chemistry
Brasilia, DF, Brazil
+55 61 3107-3096

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Our group studies the different mechanisms of neutrophil activation by bacterial products, tissue damage or contact to implant surfaces. Our major in vivo model is the systemic inflammatory response, especially the one consequent to trauma and surgery. Most of our comparative studies are based on proteomics, using different approaches, such as shotgun proteomics, targeted approaches and PTM-omics. We described neutrophil pathways affected by surgical trauma and highlighted others that are involved in the ischemic damage prevention by preconditioning.


Fontes Group



Neutrophil proteomic analysis reveals the participation of antioxidant enzymes, motility and ribosomal proteins in the prevention of ischemic effects by preconditioning.
     Arshid S, Tahir M, Fontes B, Montero EFS, Castro MS, Sidoli S, Schwämmle V, Roepstorff P, Fontes W.
     Journal of Proteomics, v. 1, p. 1, 2016. DOI: 10.1016/j.jprot.2016.05.016


High performance mass spectrometry based proteomics reveals enzyme and signaling pathway regulation in neutrophils during the early stage of surgical trauma.
     Arshid S, Tahir M, Fontes B, Montero EFS, Castro MS, Sidoli S, Roepstorff P, Fontes W.
     Proteomics Clinical Applications, cover issue 1-2/2017 doi: 10.1002/prca.201770010


Reconciling the IPC and Two-Hit Models: Dissecting the Underlying Cellular and Molecular Mechanisms of Two Seemingly Opposing Frameworks
     Morris CFM, Tahir M, Arshid S, Castro MS, Fontes W.
     J Immunol Res. 2015 doi: 10.1155/2015/697193

Proteomic Analysis of Neutrophil Priming by PAF
    Aquino EN, Dias Neves AC, Cunha KS, Uribe CE, Souza PEN, Correa JR, Castro MS, Fontes W.
    Prot. Pept. Lett. 2016 doi: 10.2174/0929866523666151202210604. 

The Neutrophil Nucleus and Its Role in Neutrophilic Function
    Carvalho LO, Aquino EN, Dias Neves AC, Fontes W.
    J Cell Biochem. 2015 Feb 28. doi: 10.1002/jcb.25124. 

Characterization of neutrophil adhesion to different titanium surfaces. 
    Campos V, Melo RCN, Silva LP, Aquino EN, Castro MS, Fontes W.
    Bulletin of Materials Science, p. 157-166, 2014. 


Evaluation of the effects of ischemic preconditioning on the hematological parameters of rats subjected to intestinal ischemia and reperfusion.
    Tahir M, Arshid S, Heimbecker AM, Castro MS, de Souza Montero EF, Fontes B, Fontes W.
    Clinics (Sao Paulo). 2015 Jan;70(1):61-8. doi: 10.6061/clinics/2015(01)11.

Comparison of the neutrophil proteome in trauma patients and normal controls.
    Teles LM, Aquino EN, Neves AC, Garcia CH, Roepstorff P, Fontes B, Castro MS, Fontes W.
    Protein Pept Lett. 2012 Jun 1;19(6):663-72.

Neutrophil proteome: lessons from different standpoints.
    Morris CF, Castro MS, Fontes W.
    Protein Pept Lett. 2008;15(9):995-1001. Review.

Proteome analysis of resting human neutrophils.
    de Souza Castro M, de Sá NM, Gadelha RP, de Sousa MV, Ricart CA, Fontes B, Fontes W.
    Protein Pept Lett. 2006;13(5):481-7.